Alumni
Postdocs & group leaders
BTU Cottbus-Senftenberg
Dr. Robert Hertel, 2020 - 2022, now @ Sartorius AG, publications.
Dr. Johannes Gibhardt, 2019 - 2020, PostDoc @ Saint Petersburg Polytechnic University (2021 - 2022), publications.
PhD students
Universität Hohenheim
Dr. Inge Schwedt, 2020 - 2023, now PostDoc @ University of Marburg, Institute for Pharmaceutical Chemistry, publications.
Georg-August-Universität Göttingen
Dr. Björn Richts, 2018 - 2021, now @ Amedes, publications.
Dr. Johannes Gibhardt, 2016 - 2019, PostDoc @ Saint Petersburg Polytechnic University (2021 - 2022), publications.
Dr. Miriam Dormeyer, 2015 - 2017, now @ Sartorius, publications.
Dr. Jonathan Rosenberg, 2015 - 2017, now @ Roche, publications.
Dr. Lorena Stannek, 2013 - 2015, now @ Evonik, publications.
Master students
University of Hohenheim
Jan Hoffmann, 2023
BTU Cottbus-Senftenberg
Syamantak Basu, 2022, publications.
Janina Berg, 2022.
Georg-August-Universität Göttingen
Lisa M. Schulz, 2019, publications.
Rica Bremenkamp, 2019, publications.
Janek Meißner, 2019, publications.
Gregor Hoffmann, 2017, publications.
Oguz Bolgi, 2017
Kah C. Yeak, 2017, publications.
Julian Schwanbeck, 2016
Johannes Gibhardt, 2015, publications.
Jonathan Rosenberg, 2014, publications.
Victoria Keidel, 2014
Richard Egelkamp, 2014, publications.
Miriam Dormeyer, 2014, publications.
Navaneethan Palanisamy, 2014
Martin Thiele, 2013, publications.
Jannik Donner, 2013
Patrick Ballin, 2012, publications.
Bachelor students
Universität Hohenheim
Franka Kemmler, 2024
Sissi Kohlen, 2024
Lara Flachsbart, 2023
Lucca Huttelmaier, 2023
Yann Kurzaj, 2023
Maria Carolina Santos (Porto), 2023
Lucia Villani, 2023
Nils Weimper, 2023
Maike Eckert, 2022, publications.
Georg-August-Universität Göttingen
Gregor Aschenbrand, 2019
Ole Hinrichs, 2018
Dennis Wicke, 2018, publications.
Jan-Philip Becker, 2016
Vivian Barz, 2015
Fabian Ott, 2015
Markus Wilkens, 2014, publications.
Johannes Gibhardt, 2013
Mirja Loreen Schulze, 2013
Richard Egelkamp, 2012
Interns
Universität Hohenheim
Manon Pizzinato (Paris), 2022, publications.
BTU Cottbus-Senftenberg
Florian Giudici (Paris), 2021, publications.
Madeline Collignon (Namur), 2021, now @ Novozymes, publications.
Prof. Dr. Andreas Kuhn (Emeritus)
Contact
Tel: 0711-459-23522
Mail
Publications
2024
136. Wenzel S, Hess R, Kiefer D, Kuhn A (2024) Involvement of the cell division protein DamX in the infection process of bacteriophage T4. Viruses 16: 487.
2023
135. Blaimschein N, Parameswaran H, Nagler G, Manioglu S, Helenius J, Ardelean C, Kuhn A, Guan L, Müller DJ (2023) The insertase YidC chaperones the polytopic membrane protein MelB inserting and folding simultaneously from both termini. Structure. 31: 1419-1430..
134. Dalbey RE, Kaushik S, Kuhn A (2023) YidC as a potential antibiotic target. BBA 1870: 119403
2022
133. Haase M, Tessmer L, Köhnlechner L, Kuhn A (2022) The M13 phage assembly machine has a membrane-spanning oligomeric ring structure. Viruses. 14: 1163.
132. Kuhn A, Thomas JA (2022) The beauty of bacteriophage T4 research: Lindsay W. Black and the T4 head assembly. Viruses. 14: 700.
131. Chen Y, Sotomayor M, Capponi S, Hariharan B, Sahu ID, Haase M, Lorigan GA, Kuhn A, White SH, Dalbey RE (2022) A hydrophilic microenvironment in the substrate-translocating groove of the YidC membrane insertase is essential for enzyme function. J Biol Chem 298: 101690.
2021
130. Laskowski PR, Pluhackova K, Haase M, Lang BM, Nagler G, Kuhn A, Müller DJ (2021) Monitoring the binding and insertion of a single transmembrane protein by an insertase. Nat Commun 12: 7082.
129. Kleinbeck F, Kuhn A (2021) Membrane insertion of the M13 minor coat protein G3p is dependent on YidC and the SecAYEG translocase. Viruses 13: 1414.
128. Steudle A, Spann D, Pross E, Shanmugam SK, Dalbey RE Kuhn A (2021) Molecular communication of the membrane insertase YidC with translocase SecYEG affects client proteins. Sci Rep 11: 3940.
127. Hariharan B, Pross E, Soman R, Kaushik S, Kuhn A, Dalbey RE (2021) Polarity/charge as a determinant of translocase requirements for membrane protein insertion. Biochim Biophys Acta Biomembr 1863: 183502.
126. Kuhn A, Leptihn S (2021) Helical and filamentous phages. Encyclopedia of Virology (4th ed.) 4: 53-60.
2020
125. Pross E, Kuhn A (2020) Two signal recognition particle sequences are present in the amino-terminal domain of the C-tailed protein SciP. J Bacteriol 203: e00312-20.
124. Wenzel S, Shneider MM, Leiman PG, Kuhn A, Kiefer D (2020) The central spike protein complex of bacteriophage T4 contacts PpiD in the periplasm of Escherichia coli. Viruses 12: 1135.
123. He H, Kuhn A, Dalbey RE (2019) Tracking the stepwise movement of a membrane-inserting protein in vivo. J Mol Biol 432: 484-496.
2019
122. Kuhn A (2019) The bacterial cell wall and membrane - a treasure chest for antibiotic targets. Subcell Biochem 92: 1-5.
121. Kuhn A (2019) Crosslinking and reconstitution approaches to study protein transport. Protein J 38: 229-235.
120. Pross E, Kuhn A (2019) The SRP signal sequence of KdpD. Sci Rep 9: 8719.
119. Serdiuk T, Steudle A, Mari SA, Manioglu S, Kaback HR, Kuhn A, Müller DJ (2019) Insertion and folding pathways of single membrane proteins guided by translocases and insertases. Sci Adv 5: eaau6824.
118. Loh B, Kuhn A, Leptihn S (2019) The fascinating biology behind phage display: filamentous phage assembly. Mol Microbiol 111: 1132-1138.
117. Dalbey R, Kuhn A, Berliner L (2019) Introduction to protein targeting and transport. Protein J 38: 199.
2018
116. Stressler T, Reichenberger K, Glück C, Leptihn S, Pfannstiel J, Swietalski P, Kuhn A, Seitl I, Fischer L (2018) A natural variant of arylsulfatase from Kluyveromyces lactis shows no formylglycine modification and has no enzyme activity. Appl Microbiol Biotechnol 102: 2709-2721.
115. Spann D, Pross E, Chen Y, Dalbey RE, Kuhn A (2018) Each protomer of a dimeric YidC functions as a single membrane insertase. Sci Rep 8: 589.
114. Ernst I, Haase M, Ernst S, Yuan S, Kuhn A, Leptihn S (2018) Large conformational changes of a highly dynamic pre-protein binding domain in SecA. Commun Biol 1: 130.
113. Kiefer D, Kuhn A (2018) YidC-mediated membrane insertion. FEMS Microbiol Lett 365: doi: 10.1093/femsle/fny106.
2017
112. Kuhn A, Haase M, Leptihn S (2017) Assisted and unassisted protein insertion into liposomes. Biophys J 113: 1187-1193.
111. Altrichter S, Haase M, Loh B, Kuhn A, Leptihn S (2017) Mechanism of the spontaneous and directional membrane insertion of a 2-transmembrane ion channel. ACS Chem Biol 12: 380-388.
110. Kuhn A, Kiefer D (2017) Membrane protein insertase YidC in bacteria and archaea. Mol Microbiol 103 590-594.
110. Kuhn A, Koch HG, Dalbey RE (2017) Targeting and insertion of membrane proteins. EcoSal Plus 7: doi: 10.1128/ecosalplus.ESP-0012-2016.
109. Loh B, Haase M, Mueller L, Kuhn A, Leptihn S (2017) The transmembrane morphogenesis protein gp1 of filamentous phage contains Walker A and Walker B motifs essential for phage assembly. Viruses 9: 73.
2016
108. Kuhn A, Dalbey RE (2016) Protein translocation: SecA-SecY conformational crosstalk opens channel. Curr Biol 26: R811-813.
107. Stressler T, Ewert J, Merz M, Funk J, Claaßen W, Lutz-Wahl S, Schmidt H, Kuhn A, Fischer L (2016) A novel glutamyl(aspartyl)-specific aminopeptidase A from Lactobacillus delbrückii with promising properties for application. PLoS One 11: e0152139.
106. Stressler T, Seitl I, Kuhn A, Fischer L (2016) Detection, production, and application of microbial arylsulfatases. Appl Microbiol Biotechnol 100: 9053-9067.
105. Stressler T, Leisibach D, Lutz-Wahl S, Kuhn A, Fischer L (2016) Homologous expression and biochemical characterization of the arylsulfatase from Kluyveromyces lactis and its relevance in milk processing. Appl Microbiol Biotechnol 100 5401-5414.
104. Pross E, Soussoula L, Seitl I, Lupo D, Kuhn A (2016) Membrane targeting and insertion of the C-tail protein SciP. J Mol Biol 428: 4218-4227.
103. Leptihn S, Gottschalk J, Kuhn A (2016). T7 ejectosome assembly: a story unfolds. Bacteriophage 6: e1128513.
2015
102. Stressler T, Eisele T, Glück C, Wangler J, Kuhn A, Fischer L (2015) Extracellular peptidases from insect- and compost-associated microorganisms: screening and usage for wheat gluten hydrolysis. European Food Research and Technology doi: 10.1007/s00217-015-2452-8.
101. Dalbey RE, Kuhn A (2015) Membrane insertases are present in all three domains of life. Structure 23: 1559-1560.
100. Lupo D, Leptihn S, Nagler G, Haase M, Molineux IJ, Kuhn A (2015) The T7 ejection nanomachine components gp15-gp16 form a spiral ring complex that binds DNA in a lipid membrane. Virology 486: 263-271.
2014
99. Dalbey RE, Kuhn A (2014) How YidC inserts and folds proteins across a membrane. Nat Struct Mol Biol 21: 435-436.
98. Kuhn A (2014) Introduction to a special issue on protein trafficking and secretion. Biochim Biophys Acta 1843: 1429-1432.
97. Seitl I, Wickles S, Beckmann R, Kuhn A, Kiefer D (2014) The C-terminal regions of YidC from Rhodopirellula baltica and Oceanicaulis alexandrii bind to ribosomes and partially substitute for SRP receptor function in Escherichia coli. Mol Microbiol 91: 408-421.
96. Dalbey RE, Kuhn A, Zhu L, Kiefer D (2014) The membrane insertase YidC. Biochim Biophys Acta 1843: 1489-1496.
95. Soman R, Yuan J, Kuhn A, Dalbey RE (2014) Polarity and charge of the periplasmic loop determine the YidC and Sec translocase requirement for the M13 procoat Lep protein. J Biol Chem 289: 1023-1032.
94. Chen Y, Soman R, Shanmugam SK, Kuhn A, Dalbey RE (2014) The role of the strictly conserved positively charged residue differs among the Gram-positive, Gram-negative, and chloroplast YidC homologs. J Biol Chem 289: 35656-35667.
2013
93. Winterfeld S, Ernst S, Börsch M, Gerken U, Kuhn A (2013) Real time observation of single membrane protein insertion events by the Escherichia coli insertase YidC. PLoS One 8: e59023.
92. Stressler T, Eisele T, Rost J, Haunschild EM, Kuhn A, Fischer L (2012) Production of polyunsaturated fatty acids by Mortiella alpina using submerse and solid state fermentation. Chem Ing Tech 85: 1-6.
2012
91. Zhu L, Klenner C, Kuhn A, Dalbey RE (2012) Both YidC and SecYEG are required for translocation of the periplasmic loops 1 and 2 of the multispanning membrane protein TatC. J Mol Biol 424: 354-367.
90. Klenner C, Kuhn A (2012) Dynamic disulfide scanning of the membrane-inserting Pf3 coat protein reveals multiple YidC substrate contacts. J Biol Chem 287: 3769-3776.
89. Quinten TA, Kuhn A (2012) Membrane interaction of the portal protein gp20 of bacteriophage T4. J Virol 86: 11107-11114.
88. Neugebauer SA, Baulig A, Kuhn A, Facey SJ (2012) Membrane protein insertion of variant MscL proteins occurs at YidC and SecYEG of Escherichia coli. J Mol Biol 417:375-386.
87. Dalbey RE, Kuhn A (2012) Protein traffic in Gram-negative bacteria -- how exported and secreted proteins find their way. FEMS Microbiol Rev 36: 1023-1045.
2011
86. Dalbey RE, Wang P, Kuhn A (2011) Assembly of bacterial inner membrane proteins. Ann Rev Biochem 80: 161-187.
85. Stiegler N, Dalbey RE, Kuhn A (2011) M13 procoat protein insertion into YidC and SecYEG proteoliposomes and liposomes. J Mol Biol 406: 362-370.
84. Ploss M, Kuhn A (2011) Membrane insertion and assembly of epitope-tagged gp9 at the tip of the M13 phage. BMC Microbiol 11: 211.
83. Imhof N, Kuhn A, Gerken U (2011) Substrate-dependent conformational dynamics of the Escherichia coli membrane insertase YidC. Biochemistry 50: 3229-3239.
82. Ernst S, Schönbauer AK, Bär G, Börsch M, Kuhn A (2011) YidC-driven membrane insertion of single fluorescent Pf3 coat proteins. J Mol Biol 412: 165-175.
2010
81. Facey SJ, Kuhn A (2010) Biogenesis of bacterial inner-membrane proteins. Cell Mol Life Sci 67: 2343-2362.
80. Wang P, Kuhn A, Dalbey RE (2010) Global change of gene expression and cell physiology in YidC-depleted Escherichia coli. J Bacteriol 192: 2193-2209.
79. Ploss M, Kuhn A (2010) Kinetics of filamentous phage assembly. Phys Biol 7: 045002.
78. Kuhn A, Stiegler N, Schubert A (2010) Membrane insertion of small proteins. Methods Mol Biol 619: 39-62.
77. Yuan R, Dalbey R, Kuhn A (2010) Membrane protein insertion in E. coli. Methods Mol Biol 619: 63-77.
76. Kreutel S, Kuhn A, Kiefer D (2010) The photosensor protein Ppr of Rhodocista centenaria is linked to the chemotaxis signalling pathway. BMC Microbiol 10: 4035.
2009
75. Benz M, Bals T, IL Gügel, Piotrowski M, Kuhn A, Schünemann D, Soll J, Ankele E (2009) Alb4 of Arabidopsis promotes assembly and stabilization of a non chlorophyll-binding photosynthetic complex, the CF1CF0-ATP synthase. Mol Plant 2: 1410-1424.
74. Kuhn A (2009) From the Sec complex to the membrane insertase YidC. Biol Chem 390: 701-706.
73. Winterfeld S, Imhof N, Roos T, Bär G, Kuhn A, Gerken U (2009) Substrate-induced conformational change of the Escherichia coli membrane insertase YidC. Biochemistry 48: 6684-6691.
2008
72. Maier KS, Hubich S, Liebhart H, Krauss S, Kuhn A, Facey SJ (2008) An amphiphilic region in the cytoplasmic domain of KdpD is recognized by the signal recognition particle and targeted to the Escherichia coli membrane. Mol Microbiol 68: 1471-1484.
71. Gerken U, Erhardt D, Bär G, Ghosh R, Kuhn A (2008) Initial binding process of the membrane insertase YidC with its substrate Pf3 coat protein is reversible. Biochemistry 47: 6052-6058.
70. Klenner C, Yuan J, Dalbey RE, Kuhn A (2008) The Pf3 protein contacts TM1 and TM3 of YidC during membrane biogenesis. FEBS Lett 582: 3967-3972.
2007
69. Nagler C, Nagler G, Kuhn A (2007) Cysteine residues in the transmembrane regions of M13 procoat protein suggest that oligomeric coat proteins assemble onto phage progeny. J Bacteriol 189: 2897-2905.
68. Facey SJ, Neugebauer SA, Krauss S, Kuhn A (2007) The mechanosensitive channel protein MscL is targeted by the SRP to the novel YidC membrane insertion pathway of Escherichia coli. J Mol Biol 365: 995-1004.
67. Kiefer D, Kuhn A (2007) YidC as an essential and multifunctional component in membrane protein assembly. Int Rev Cytol 259: 118-138.
2006
66. Xie K, Kiefer D, Nagler G, Dalbey RE, Kuhn A (2006) Different regions of the nonconserved large periplasmid domain of Escherichia coli YidC are involved in the SecF interaction and membrane insertase activity. Biochemistry 45: 13401-13408.
65. Celebi N, Yi L, Facey SJ, Kuhn A, Dalbey RE (2006) Membrane biogenesis of subunit II of cytochrome bo oxidase contrasting requirements for insertion of N-terminal and C-terminal domains. J Mol Biol 357: 1428-1436.
64. Aisenbrey C, Harzer U, Bauer-Manz G, Bär G, Husnal Chotimah IN, Bertani P, Sizun C, Kuhn A, Bechinger B (2006) Proton-decoupled 15N and 31P solid-state NMR investigations of the Pf3 coat protein in oriented phospholipid bilayers. FEBS J 273: 817-828.
63. Rothenbüchner MC, Facey SJ, Kiefer D, Kossmann M, Kuhn A (2006) The cytoplasmic C-terminal domain of the Escherichia coli KdpD protein functions as a K+ sensor. J Bacteriol 188: 1950-1958.
2005
62. Papanikou E, Karamanou S, Baud C, Frank M, Sianidis G, Keramisanou D, Kalodimos CG, Kuhn A, Economou A (2005) Identification of the preprotein binding domain of SecA. J Biol Chem 280: 43209-43217.
61. Chen M, Xie K, Yuan J, Yi L, Facey SJ, Pradel N, Wu LF Kuhn A, Dalbey RE (2005) Involvement of SecDF and YidC in the membrane insertion of M13 procoat mutants. Biochemistry 44: 10741-10749.
60. Baud C, Papanikou E, Karamanou S, Sianidis G, Kuhn A, Economou A (2005) Purification of a functional mature region from a SecA-dependent preprotein. Protein Expr Purif 40: 336-339.
2004
59. Serek J, Bauer-Manz G, Struhalla G, van den Berg L, Kiefer D, Dalbey R, Kuhn A (2004) Escherichia coli YidC is a membrane insertase for Sec-dependent proteins. EMBO J 23: 294-301.
58. Facey SJ, Kuhn A (2004) Membrane integration of E. coli model membrane proteins. Biochim Biophys Acta 1694: 55-66.
57. Dalbey RE, Kuhn A (2004) YidC family members are involved in the membrane insertion, lateral integration, folding, and assembly of membrane proteins. J Cell Biol 166: 769-774.
2003
56. Jiang F, Chen M, Yi L, de Gier JW, Kuhn A, Dalbey RE (2003) Defining the regions of Escherichia coli YidC that contribute to activity. J Biol Chem 278: 48965-48965.
55. Kuhn A, Stuart R, Henry R, Dalbey RE (2003) The Alb3/Oxa1/YidC protein family: membrane-localized chaperones facilitating membrane proteins insertion? Trends Cell Biol 13: 510-516.
54. Facey S, Kuhn A (2003) The sensor protein KdpD inserts into the Escherichia coli membrane independent of the Sec translocase and YidC. Eur J Biochem 270: 1724-1734.
2002
53. Chen M, Samuelson JC, Jiang F, Muller M, Kuhn A, Dalbey RE (2002) Direct interaction of YidC with the Sec-independent Pf3 coat protein during its membrane protein insertion. J Biol Chem 277: 7670-7675.
52. Kuhn A, Spiess M (2002) Membrane protein insertion into bacterial membranes and the endoplasmic reticulum. Protein targeting, transport and translocation. Acad. Press - Amsterdam (Ed. R. Dalbey, G. von Heijne). pp. 107-130.
51. Ridder ANJA, van den Hoef W, Stam J, Kuhn A, Killian A, de Kruijff B (2002) Importance of hydrophobic matching for spontaneous insertion of single-spanning membrane protein. Biochemistry 41: 4946-4952.
2001
50. Ridder ANJA, Kuhn A, Killian JA, de Kruijff B (2001) Anionic lipids stimulate Sec-independent insertion of a membrane protein lacking charged amino acid side chains. EMBO Rep 2: 403-408.
49. Eisenhawer M, Cattarinussi S, Kuhn A, Vogel H (2001) Fluorescence energy transfer shows a close helix-helix distance in the transmembrane M13 procoat protein. Biochemistry 40: 12321-12328.
48. Samuelson JC, Jiang F, Yi L, Chen M, Kuhn A, Dalbey RE (2001) Function of YidC for the insertion of M13 procoat protein in E. coli: translocation of mutants that show differences in their membrane potential dependence and Sec-recruitment. J Biol Chem 276: 34847-34852.
47. Roos T, Kiefer D, Hugenschmidt S, Economou A, Kuhn A (2001) Indecisive M13 procoat mutants bind to SecA but do not activate the translocation ATPase. J Biol Chem 276: 37909-37915.
2000
46. Dalbey RE, Kuhn A (2000) Evolutionarily related insertion pathways of bacterial, mitochondrial, and thylakoid membrane proteins. Annu Rev Cell Dev Biol 16: 51-87.
45. Ridder ANJA, Morein S, Stam JG, Kuhn A, de Kruijff B, Killian JA (2000) Analysis of the role of interfacial tryptophan residues in controlling the topology of membrane proteins. Biochemistry 39: 6521-6528.
44. Samuelson JC, Chen M, Jiang F, Möller M, Wiedmann M, Kuhn A, Phillips GJ, Dalbey RE (2000) YidC mediates membrane protein insertion in bacteria. Nature 406: 637-641.
1999
43. Karamanou S, Vrontou E, Sianidis G, Baud C, Roos T, Kuhn A, Politou AS, Economou A (1999) A molecular switch in SecA protein couples ATP hydrolysis to protein translocation. Mol Microbiol 34: 1133-1145.
42. Kiefer D, Kuhn A (1999) Hydrophobic forces drive the spontaneous membrane insertion of the bacteriophage Pf3 coat protein without topological control. EMBO J 18: 6299-6306.
1998
41. de Gier JW, Scotti PA, Sääf A, Valent QA, Kuhn A, Luirink J, von Heinje G (1998) Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli. Proc Natl Acad Sci USA 95: 14646-14651.
40. Kiefer D, Kuhn A (1998) Insertion of single and multi-spanning proteins into the bacterial cytoplasmic membrane. Protein Targeting and Translocation. Portland Press. pp. 67-84.
1997
39. Kiefer D, Hu X, Dalbey R, Kuhn A (1997) Negatively charged amino acid residues play an active role in orienting the Sec-independent Pf3 coat protein in the Escherichia coli inner membrane. EMBO J 16: 2197-2204.
1996
38. Soekarjo M, Eisenhawer M, Kuhn A, Vogel H (1996) Thermodynamics of the membrane insertion process of the M13 procoat protein, a lipid bilayer traversing protein containing a leader sequence. Biochemistry 35: 1232-1241.
1995
37. Kuhn A (1995) Major coat proteins pf bacteriophage Pf3 and M13 as model systems for Sec-indpendent protein transport. FEMS Microbiol Rev 17: 185-190.
36. Dalbey RE, Kuhn A, von Heinje G (1995) Directionality in protein translocation across membranes: the N-tail phenomenon. Trends Cell Biol 5: 380-383.
35. Kiefer D, Kuhn A (1995) Membrane insertion of small proteins: evolutionary and functional aspects. Adv Cell Mol Biol Membr Org 4: 17-33.
34. Cao G, Kuhn A, Dalbey RE (1995) The translocation of negatively charged residues across the membrane is driven by the electrochemical potential: evidence for an electrophoresis-like membrane transfer mechanism. EMBO J 14: 866-875.
1994
33. Kusters R, Breukink E, Galluser A, Kuhn A, de Kruijff B (1994) A dual role for phosphatidylglycerol in protein translocation across the Escherichia coli inner membrane. J Biol Chem 269: 1560-1563.
32. Kuhn A, Kiefer D, Köhne C, Zhu HY, Tschantz WR, Dalbey RE (1994) Evidence for a loop-like insertion mechanism of pro-Omp A into the inner membrane of Escherichia coli. Eur J Biochem 226: 891-897.
31. Pawlak M, Kuhn A, Vogel H (1994) Pf3 coat protein forms voltage-gated ion channels in planar lipid bilayers. Biochemistry 33: 283-290.
30. Cao G, Cheng S, Whitley P, von Heinje G, Kuhn A, Dalbey RE (1994) Synergistic insertion of two hydrophobic regions drives Sec-independent membrane protein assembly. J Biol Chem 269: 26898-26903.
1993
29. Geller B, Zhu HY, Cheng S, Kuhn A, Dalbey RE (1993) Charged residues render pro-OmpA potential dependent for initiation of membrane translocation. J Biol Chem 268: 9442-9447.
28. Thiaudere E, Soekarjo M, Kuchinka E, Kuhn A, Vogel H (1993) Structural characterization of membrane insertion of M13 procoat, M13 coat, and Pf3 coat proteins. Biochemistry 32: 12186-12196.
1992
27. Hintermann E, Kuhn A (1992) Bacteriophage T4 gene 21 encodes two proteins essential for phage maturation. Virology 189: 474-482.
26. Lee JI, Kuhn A, Dalbey RE (1992) Distinct domains of an oligotopic membrane protein are Sec-dependent and Sec-independent for membrane insertion. J Biol Chem 267: 938-943.
25. Kuhn A, Troschel D (1992) Distinct steps in the insertion pathway of bacteriophage coat proteins. New Comprehensive Biochemistry 22: 33-47.
1991
24. Shen LM, Lee JI, Cheng SY, Jutte H, Kuhn A, Dalbey RE (1991) Use of site-directed mutagenesis to define the limits of sequence variation tolerated for processing of the M13 procoat protein by Escherichia coli leader peptidase. Biochemistry 30: 11775-11781.
1990
23. Kuhn A, Rohrer J, Gallusser A (1990) Bacteriophage M13 and Pf3 tell us how proteins insert into membrane. J Struct Biol 104: 38-43.
22. Kuhn A, Zhu HY, Dalbey RE (1990) Efficient translocation of positively charged residues of M13 procoat protein across the membrane excludes electrophoresis as the primary force for membrane insertion. EMBO J 9: 2385-2389.
21. Gallusser A, Kuhn A (1990) Initial steps in protein membrane insertion. Bacteriophage M13 procoat protein binds to the membrane surface by electrostatic interaction. EMBO J 9: 2723-2729.
20. Rohrer J, Kuhn A (1990) The function of a leader peptide in translocating charged amino acyl residues across a membrane. Science 250: 1418-1421.
1988
19. Kuhn A (1988) Alterations in the extracellular domain of M13 procoat protein make its membrane insertion dependent on secA and secY. Eur J Biochem 177: 267-271.
1987
18. Dalbey RE, Kuhn A, Wickner W (1987) The internal signal sequence of Escherichia coli leader peptidase is necessary but not sufficient for its rapid membrane assembly. J Biol Chem 262: 13241-13245.
17. Kuhn A (1987) Bacteriophage M13 procoat protein inserts into the plasma membrane as a loop structure. Science 238: 1413-1415.
16. Kuhn A, Keller B, Maeder M, Traub F (1987) The prohead core of bacteriophage T4 can act as an intermediate in the T4 assembly pathway. J Virol 61: 113-118.
15. Kuhn A, Krell G, Wickner W (1987) Recombinant forms of M13 procoat with an OmpA leader sequence of a large carboxyterminal extension retain their independence of secY function. EMBO J 6: 501-505.
14. Dalbey RE, Kuhn A, Kreil G, Rice MC, Wickner W (1987) Mechanism of membrane assembly and protein secretion. Chemica Scripta i27B: 245-248.
1986
13. Dalbey RE, Kuhn A, Wickner W (1986) Leader peptidase from Escherichia coli. In Microbiology 1986, ASM, Washington DC. pp. 234-237.
12. Kuhn A, Wickner W, Kreil G (1986) The cytoplasmic carboxy-terminus is required for the membrane insertion of the central domain of M13 procoat. Nature 322: 335-339.
11. Kuhn A, Krell G, Wickner W (1986) Both hydrophobic domains of M13 procoat initiate membrane insertion. EMBO J 5: 3681-3685.
1985
10. Alatossava T, Jütte H, Kuhn A, Kellenberger E (1984). Manipulation of intracellular magnesium content in polymyxin B nonapeptide-sensitized Escherichia coli by ionophore A23187. J Bacteriol 162: 413-419.
9. Kuhn A, Kellenberger E (1985) Productive phage infection can occur in E. coli with drastically reduced internal levels of cations. J Bacteriol 163: 906-912.
8. Kuhn A, Wickner W (1985) Isolations of mutants in M13 coat protein that affect its synthesis, processing and assembly into phage. J Biol Chem 260: 15907-15913.
7. Kuhn A, Wickner W (1985) Conserved residues of the leader peptide are essential for cleavage by leader peptidase. J Biol Chem 260: 15914-15918.
1984
6. Kuhn A, Seiler HG (1984) The discrimination between Rb+ and K+ by Escherichia coli is changed after bacteriophage T7 infection. Biochem Biophys Acta 771: 245-248.
5. Traub F, Keller B, Kuhn A, Maeder M (1984) Isolation of the prohead core of bacteriophage T4 after crosslinking and determination of protein composition. J Virol 49: 902-908.
1983
4. Kuhn A, Jütte H, Kellenberger E (1983) Involvement of the bacterial groM gene product in bacteriophage T7 reproduction. II. A reduced level of ion concentrations causes block of T7 maturation in K-12 cells. J Virol 47: 540-552.
1982
3. Völker TA, Kuhn A, Showe MK, Bickle TA (1982) Gene 67: a new essential bacteriophage T4 head gene codes for a core component, PIP. II. The construction in vitro of unconditionally lethal mutants and their maintenance. J Mol Biol 161: 491-504.
2. Kuhn A. (1982) Consequences on phage development by changes of intracellular ionic concentrations: studies on different virus host systems. University Microfilms Int., Ann Arbor, MI 48106, USA.
1. Kuhn A, Moncany M, Hausmann R, Kellenberger E (1982) Involvement of the bacterial groM gene product in bacteriophage T7 reproduction. I. Arrest at the level of DNA packaging. J Virol 41: 657-673.